Design, Synthesis and Applications of Fluorescent and Electrochemical Probes

................................................................................................................... 46 Introduction .............................................................................................................. 47 5 Experimental section ................................................................................................ 49 2.3.1 Materials ............................................................................................................. 49 2.3.2 NOC-7 treatment and fluorescence imaging ....................................................... 49 2.3.3 LPS treatment and fluorescence imaging ........................................................... 50 Results and Discussion ............................................................................................ 50 2.4.1 Synthetic approach to fluorescent probe for NO ................................................ 50 2.4.2 Optical properties of BODIPY dyes ................................................................... 51 2.4.3 The fluorescent response and selectivity of the fluorescent probe A to NO ...... 53 2.4.4 Use of fluorescent probe to detect nitric oxide in living cells ............................ 56 Conclusion ............................................................................................................... 60 Abstract .................................................................................................................... 68 Introduction .............................................................................................................. 68 Experimental Section ............................................................................................... 70 3.3.1 Instrumentation. .................................................................................................. 70 3.3.2 Materials ............................................................................................................. 71 3.3.3 Cell culture and confocal fluorescence imaging ................................................. 74 Results and Discussion ............................................................................................ 75 3.4.1 Design and synthesis of near-infrared fluorescent probes for pH ....................... 75 3.4.2 Optical responses of fluorescent probes to pH ................................................... 76 3.4.3 The selectivity experiments of fluorescent probes to pH over metal ions. ......... 80 3.4.4 Live cell imaging of fluorescent probes .............................................................. 81 6................................................................................................................... 68 Introduction .............................................................................................................. 68 Experimental Section ............................................................................................... 70 3.3.1 Instrumentation. .................................................................................................. 70 3.3.2 Materials ............................................................................................................. 71 3.3.3 Cell culture and confocal fluorescence imaging ................................................. 74 Results and Discussion ............................................................................................ 75 3.4.1 Design and synthesis of near-infrared fluorescent probes for pH ....................... 75 3.4.2 Optical responses of fluorescent probes to pH ................................................... 76 3.4.3 The selectivity experiments of fluorescent probes to pH over metal ions. ......... 80 3.4.4 Live cell imaging of fluorescent probes .............................................................. 81 6